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Detergent selection for enhanced extraction of membrane ...- dm détergent anatrace ,Nov 01, 2012·Highlights Detergent efficiencies were examined for the extraction of membrane proteins. Extraction efficiencies varied with different classes of membrane proteins. Higher detergent concentrations increased extraction efficiencies but also led to protein denaturation. The most efficient detergents were correlated with different types of membrane proteins.膜蛋白提取去垢剂DDM990--十二烷基-β-D ... - 简书十二烷基-β-D-麦芽糖苷 (N-Dodecyl-β-D-maltoside, DDM)，是一种非离子型去垢剂，属于烷基糖苷类非离子型表面活性剂。. DDM常用来抽提和溶解蛋白质，稳定酶活性和激活酶以及膜研究。. DDM优势：DDM高效分离保留活性的疏水性膜蛋白，优于CHAPS (3-环乙胺-1-丙磺酸)或NP-40 ...
https:///result/decyl maltoside detergent dm/product/Anatrace Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99 decyl maltoside detergent dm - by Bioz Stars , 2021-12 86 / 100 stars Buy from Supplier n decyl β d maltoside ( Anatrace ) 86 Anatrace n decyl β d maltoside
Jan 01, 2021·Among other parameters, detergent selection should consider solubilization efficacy and the effects on protein stability. Moreover, the size of formed micelles is important, dictating the overall size of protein-detergent complexes that can be vital for the downstream applications, e.g., crystallization (Prive, 2007; Tate, 2010) or cryo-EM (Thonghin et al., 2018).
Jan 01, 2009·The detergent concentration needed depends on how it is being used. A higher concentration is needed for dissolving the membranes, and the more membranes per volume of detergent solution the ...
[2] Anatrace, Detergents and Their Uses in Membrane Protein Science. [3] M. le Maire, P. Champeil, J.V. Mbller, Interaction of membrane proteins and lipids with solubilizing detergents, Biochim. Biophys.Acta 1508 (2000) 86–111. [4] From Wikipedia, the free encyclopedia. [5] Purifying Challenging Proteins Principles and Methods, 28-9095-31.
DETERGENTS AND THEIR USES IN MEMBRANE PROTEIN SCIENCE Nonionic detergents, including maltosides, glucosides, and polyoxyethylene glycols are characterized by uncharged hydrophilic head groups. These detergents are mild and nondenaturing because they disrupt protein-lipid and lipid-lipid interactions rather than protein-protein interactions.
Jul 17, 2019·The most commonly used detergent families in membrane protein crystallography are maltosides and glucosides, followed by amine oxides and polyoxyethylene glycols 3. Altogether, crystallographic data support the use of n-Dodecyl β-D-maltoside (DDM), n-Decyl-β-D-Maltopyranoside (DM), Octyl-beta-glucoside (OG) and Lauryldimethylamine-N-oxide (LDAO).
Nov 01, 2010·The dried lipid films were hydrated and solubilized in 2 mL of 2–6% β-octylglucoside (Anatrace, Anagrade), 20 mM 3-(N-morpholino)propanesulfuric acid (MOPS), 200 mM NaCl (pH 7.5). The SNGpATM stock solution in DM was added to the solubilized POPC at a final concentration of 10 μM and incubated at room temperature for 1 h.
Mar 09, 2022·GDN is effective for structural studies of GPCRs as well as non-GPCRs; this detergent has facilitated structure elucidation of 58 GPCRs and 138 non-GPCR including 59 channels, 34 transporters, 26 enzymes, and 19 others ( Figure 6 B). Thus, GDN is suitable for structural studies of diverse classes of membrane proteins.
Non-ionic detergent for the stabilization and activation of enzymes and for membrane research. n-Dodecyl β-D-maltoside has been used in a study to assess the mapping of unfolding states of integral helical membrane proteins by GPS-NMR and scattering techniques. Packaging.
50 ml. 356. Units. Add to cart. Product Description Technical Documentation Required / Related Products. Formulation components – Dodecyl Maltoside (D310) and Cholesteryl Hemisuccinate (CH210) Formulation -. o 10:1 Dodecyl Maltoside to Cholesteryl Hemisuccinate (CH210) in deionized water. o Final concentration 10% Dodecyl Maltoside (D310) to ...
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The micelle sizes of C 12 E 9 and FOS14 detergents have not been measured previously (Anatrace, pers. comm.), and are 83 ± 1.6 kDa (C 12 E 9), and 47 ± 1.2 kDa (FOS14) in buffer containing 25 mM Tris pH 8.0 and 100 mM NaCl (Table 1 1).). The size of some detergent micelles might be dependent on concentration and temperature (Corti et al. 1984).
Information about the Detergent Database. Year: Select... 1995 1996 1997. Classification: Select... Alpha Helical Beta Barrel Monotopic. Solubilization …
overconcentration of a detergent (in free micelles), a potentially harmful event for crystallization [12]. The higher concentration of a detergent, the higher the chance of phase separation, which might ultimately lead to protein denaturation. Parameters such as temperature, salt, and precipitant also influence the detergent phase behavior [13 ...
overconcentration of a detergent (in free micelles), a potentially harmful event for crystallization [12]. The higher concentration of a detergent, the higher the chance of phase separation, which might ultimately lead to protein denaturation. Parameters such as temperature, salt, and precipitant also influence the detergent phase behavior [13 ...
n-dodecyl-β-D-maltopyranoside. DDM is the most widely used detergent for the isolation and purification of GPCR’s. In addition, LacY likely favors a higher-energy intermediate periplasmic-open conformation in situ, but collapses to a lower-energy periplasmic-closed conformation in DDM after purification. It is interesting to note that the ...
Mar 16, 2021·The obtained IMAC elution profile for hAQP10 extracted with t-PCCαM (Fig. 2 a, red) shares high similarity with the one observed for DM (Fig. S2b), the detergent that was previously used for solubilization and affinity purification of the hAQP10 sample used for crystallization (Gotfryd et al., 2018b).
Jun 15, 2011·To investigate the effect the detergent concentrations on solution phase characteristics, colloidal solutions of PS I in 200 mM sodium phosphate buffer (pH = 7.0) were prepared with PS I concentrations of 7.2 × 10 −5 mM in buffer solutions stabilized with different concentrations of the non-ionic detergents, DM and TX-100 with respect to their respective …
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Dec 01, 2009·Four detergents (DM, DDM, DPC, LDAO) were analyzed using GC–MS. Detergents eluted from the GC column were ionized through electron impact ionization (EI) and a total ion current (TIC) chromatogram contained peaks at different retention time, representing different detergents (Fig. 1A and D).A specific fragment ion for each detergent (i.e. large m/z …
Nov 01, 2012·The detergents used in this study were provided by Anatrace. Plasmids and cell growth. Escherichia coli cell lines (C41, C43, BL21(DE3) ... In contrast, decyl-β-d-maltoside (DM), another alkyl sugar detergent, and the other non-ionic specialty detergents extracted less than half of these total protein levels, while dodecyldimethylamine-N-oxide ...
Packing parameters are assigned to detergent monomers and are useful for predicting the shape of the aggregate (i.e., spherical or lamellar) formed by those monomers. For example, P<1/3 indicates that the detergent will likely form spherical micelles while 1/3<P<1/2 indicates that the detergent will likely form cylindrical micelles [25].
Which detergent is better to use for stabilizing the protein and retaining its function? ... DM, OG, LMNG etc. ... The description from Anatrace doesn't really say much except that it's soluble to ...
Aug 18, 2016·Detergent Properties Detergent Analysis CMC - Anatrace